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2	Overview What is the Adirondack ATBI Initiative Two parts to the biodiversity inventory: Science Citizen participation Upcoming ATBI Activities and Opportunities
3	Core Traits of Adirondack ATBI: All Adirondacks All taxonomic groups Public and private collaboration Strong citizen participation Education role
4	Mission Survey the diversity of life and connect people to the natural world through participation in biological inventories and related activities in the Adirondack Park of New York State.
5	Biodiversity Inventory
6	Ecosystem Framework Stratified Standardized Sampling Protocols Spatially-referenced Direct future analyses
7	Taxonomic Working Groups (TWIGS)
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9	Discovery
10	Citizen Participation
11	Appreciation of Place-Based Biodiversity through Art, Culture and Community
12	Activities Discover the Dragonflies July 29, Newcomb VIC August 19, Paul Smiths VIC September 15, NHMA Wild Center (K-12) Other Aug 9 ATBI talk Paul Smiths VIC July 30-Aug 4 Aquatic Plant Workshop, Paul Smiths College Mid-October GPS Workshop, Adirondack Ecological Center
13	Getting Involved Field Inventory Volunteer Lead sampling for your taxon of choice! Committees Science – organize Taxonomic Working Groups Community Outreach – get message out, organize volunteers Education – coordinate with schools, hold training workshops
14	On the Cutting Edge of Biotechnology: DNA Barcoding and the ATBI
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16	Biodiversity studies are limited by availability of taxonomists 1.7 million species of plants and animals are known There could be as many as 10 million or even 100 million species on the planet
17	"DNA barcoding is the idea..." DNA barcoding is the idea that a short genetic sequence is enough to identify nearly every species
18	What DNA sequence has the right level of variability to serve as a species identifier? The sequence of the cytochrome oxidase I gene is just right in terms of variability to serve as a species biomarker
19	"The DNA “barcode" The DNA “barcode” is a sequence of about 650 DNA letters of the mitochondrial COI gene.
20	"an international initiative devoted to..." an international initiative devoted to developing DNA barcoding as a global standard in taxonomy CBOL has more than 100 member organizations from 40 countries
21	How does barcoding work? DNA sequence from an organism is obtained Sequence data is entered into a large, public-access database The “best match” to the input sequence provides species identification
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23	Successes in the classroom In a local classroom, Schroon Lake high school teacher, Cookie Barker, has performed genetic analysis of the deer population using mitochondrial genes Her classroom has processed hundreds of deer and brook trout samples for analysis
24	"The ATBI can contribute to" The ATBI can contribute to, use and test the barcoding system
25	DNA barcoding may allow the identification of cryptic species A butterfly, Astraptes fulgerator, was recently found, through DNA barcoding, to actually be comprised of 16 different species all of which look identical as adults
26	"DNA barcoding allows us to..." DNA barcoding allows us to identify species at any life stage and even if the organism is not there in it’s entirety
27	"DNA barcoding has been used..." DNA barcoding has been used to estimate diversity of stomatopods at the planktonic, larval stage
28	"Is this an Allen’s or..." Is this an Allen’s or Rufus hummingbird? The species id was made using a single feather found under a feeder
29	Nano-scale DNA sequencing Microfabricated bioprocessor for integrated nanoliter-scale DNA sequencing (reported in May 9, 2006 PNAS)
30	Convinced? How do we start?
31	It’s all about the toys?
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43	"http://library.paulsmiths.edu/ATBI/index.htm" http://library.paulsmiths.edu/ATBI/index.htm